جامعة تكريت - كلية الطب البيطري - جامعة تكريت

EVALUATION OF TWO LOCAL ISOLATED INFECTIOUS BURSAL DISEASE VIRUS WITH COMMERCIAL VACCINAL

STRAINS IN BROILER

Salah M. Hassan , Mohammed K. Shakor

Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Baghdad , Iraq  

عنوان البريد الإلكتروني هذا محمي من روبوتات السبام. يجب عليك تفعيل الجافاسكربت لرؤيته. عنوان البريد الإلكتروني هذا محمي من روبوتات السبام. يجب عليك تفعيل الجافاسكربت لرؤيته.     

عنوان البريد الإلكتروني هذا محمي من روبوتات السبام. يجب عليك تفعيل الجافاسكربت لرؤيته. عنوان البريد الإلكتروني هذا محمي من روبوتات السبام. يجب عليك تفعيل الجافاسكربت لرؤيته.

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Abstract

This study was conducted to evaluate the best vaccination program by using commercial vaccinal strains (IBDL and E228) and two local isolated strains (first experiment groups were vaccinated at 14 days) while (second experiment groups were vaccinated at 21 days). Five hundred broiler chicks (Ross-308) from AL-Anwar hatchery/Tikrit province were divided randomly into five groups each group (n= 50) chicks in each experimental groups  and treated as follow:-

Group 1: Vaccinated with Commercially IBD (Ceva- IBDL Hungary strain EID₅₀ 10^2.3) in drinking water. Group 2: Vaccinated with Commercially IBD (Intervet E228IBD strain EID₅₀ 10²) in drinking water. Group 3: Vaccinated with Local IBD isolated strain (CH IBD 2013 M1 Baghdad EID₅₀ 10^7.3) vaccine in drinking water. Group 4: Vaccinated with Local IBD isolated strain (CH IBD 2013 M2 Tikrit EID₅₀ 10^5.4) in drinking water. Group 5: (control group) was not vaccinated without any vaccine.

Blood samples were collected in both experiments from the jugular vein at1, 7, 14, 21, 28, 35 and 40 days to determine the antibody titer against IBDV by ELISA, tissue samples were also taken from the bursa for Quantitive Real-time (RT)-PCR (viral load) at 2 and 4 days post vaccination.

The results of two experiment above revealed that G3 showed a high significant differences (P<0.05) in protection against IBD isolate (CH IBD 2013 M1 Baghdad EID₅₀ 10^7.3) compared with other groups. The ELISA results of serum analysis showed significant increase (P<0.05) in antibody titer against IBDV especially in second experiment when vaccinated at day 21 that revealed low maternal immunity. On the other hand, RNA copies of the IBDV in the bursal tissues determined by quantitative Real - time reverse transcriptase PCR (RT- qPCR) showed increase viral load in second experiment in comparison with first group that showed decrease RNA copies due to neutralization with maternal immunity.

Key word: infectious bursal disease, isolate,broilers, ELISA, viral load.