جامعة تكريت - كلية الطب البيطري - جامعة تكريت

Efficacy and histopathological study of bile salts against hydatidosis comparing with antigen B in experimentally infected mice.

Inam Faleh¹ , Layla  Hashim¹ ,  Hawraa M. Murad²

¹ college of veterinary medicine , Baghdad University , Baghdad , Iraq

² college of veterinary  medicine , Al Qassim Green University , Baghdad , Iraq

Email: عنوان البريد الإلكتروني هذا محمي من روبوتات السبام. يجب عليك تفعيل الجافاسكربت لرؤيته. عنوان البريد الإلكتروني هذا محمي من روبوتات السبام. يجب عليك تفعيل الجافاسكربت لرؤيته.

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Abstract

The present study was carried out to investigate some immunopathological effects of bovine bile salts and antigen (AgB) of hydatid cyst fluid on the immune response against hydatid cyst infection in mice. In order to achieve this goal Antigen B of hydatid cyst fluid was prepared from fertile sheep hydatid cyst according to oral method by using the (Ion Exchange Chromatography), thirty white mice of both sexes that aged 4-8weeks were divided randomly into four groups as below:

The 1st group of 10 mice were used for oral immunization with 0.3ml that contained 2mg/ml of bile salt with 14 days intervals, The 2^nd group of 10 mice were immunized subcutaneously(s/c) with Antigen B of hydatid cyst fluid -with 0.3ml AgB that contained 3.1mg/ml of protein concentration by two doses with 14days intervals.

The 3^rdgroup of 10 mice was inoculated intraperitonally with 1ml that contained 2000 protoscolices and served as control positive, 1^st, 2^nd and 3^rdgroups were challenged intraperitonailly with 2000 protoscolices (psc), then post mortem examination was made after 3months post challenged and specimens of visceral organs were fixed with 10%formalin for the histopathological studies.   

Severe histopathological lesions were recorded in the control positive group that characterized by the development of fertile hydatid cyst structure in the liver parenchyma with abscess formation in the liver, spleen and kidney amyloidosis, while  the main histopathological findings in the immunized groups revealed multiple and focal MNCs infiltration with lymphoid hyperplasia mainly in the bile salts immunized groups together with granulomatous inflammation reaction that seen mainly in the liver and kidney as well as brain perivascular cuffing. In conclusion the treated animals with oral administration of bile salts induced potential, protective immune response against experimental murine hydatidosis in comparison with E.g AgB that were immunized intragastrically by oral gavage with 0.3 ml (2mg/ml) of bovine bile salts.

Keyword: Echinococcus  granulosus, Bile salts, Mice.