جامعة تكريت - كلية الطب البيطري - جامعة تكريت

A new cell line in Cryptosporidium spp. in vitro culturing 

Hiro M. Obiad¹ , Tawfiq I.Al-Alousi² , Abdulla H.Al-Jboori³ 

¹ Medical  Lab. Techniques  dept. , College of Technology – Kirkuk ,  Foundation of Technical Education , Kirkuk , Iraq

² Microbiology Dept. , Veterinary Medicine College , Tikrit University , Tikrit , Iraq

³ Biology Dept. , Education College , Tikrit University , Tikrit , Iraq

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Abstract

This study was designed to find out the ability of Cryptosporidium spp. to grow and  develop in  stem cells from human placenta. Because this could have serious transmission implications in relation to pregnant human and other vertebrate hosts especially the immune compromised one. Cryptosporidium was cultured successfully in human placental stem cells from (chorion, amnion, umbilical cord), and mice bone marrow stem cells, with rate of 22, 20, 16, 10 %  respectively for each one, comparing with 24 %  in mice peritoneal macrophages. Both sexual and a sexual reproductions was occurred in cultured stem cells and the produced oocysts were seen free within the culture media. This result is represent the first time for recording the parasite growth in placental stem cells and this may open a gate for more intracellular parasites culturing and promise a source of available and abundant undifferentiated  cells for other researches.

Key words: Cell culture, Human placenta, Cryptosporidium.